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Knit directory: damsel_paper/analysis/
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library(Damsel)
library(plyranges)
Loading required package: BiocGenerics
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
anyDuplicated, aperm, append, as.data.frame, basename, cbind,
colnames, dirname, do.call, duplicated, eval, evalq, Filter, Find,
get, grep, grepl, intersect, is.unsorted, lapply, Map, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
Position, rank, rbind, Reduce, rownames, sapply, setdiff, table,
tapply, union, unique, unsplit, which.max, which.min
Loading required package: IRanges
Warning: package 'IRanges' was built under R version 4.4.1
Loading required package: S4Vectors
Warning: package 'S4Vectors' was built under R version 4.4.1
Loading required package: stats4
Attaching package: 'S4Vectors'
The following object is masked from 'package:utils':
findMatches
The following objects are masked from 'package:base':
expand.grid, I, unname
Loading required package: GenomicRanges
Loading required package: GenomeInfoDb
Attaching package: 'plyranges'
The following object is masked from 'package:IRanges':
slice
The following object is masked from 'package:stats':
filter
library(dplyr)
Attaching package: 'dplyr'
The following objects are masked from 'package:plyranges':
between, n, n_distinct
The following objects are masked from 'package:GenomicRanges':
intersect, setdiff, union
The following object is masked from 'package:GenomeInfoDb':
intersect
The following objects are masked from 'package:IRanges':
collapse, desc, intersect, setdiff, slice, union
The following objects are masked from 'package:S4Vectors':
first, intersect, rename, setdiff, setequal, union
The following objects are masked from 'package:BiocGenerics':
combine, intersect, setdiff, union
The following objects are masked from 'package:stats':
filter, lag
The following objects are masked from 'package:base':
intersect, setdiff, setequal, union
library(tidyr)
Attaching package: 'tidyr'
The following object is masked from 'package:S4Vectors':
expand
library(edgeR)
Warning: package 'edgeR' was built under R version 4.4.1
Loading required package: limma
Warning: package 'limma' was built under R version 4.4.1
Attaching package: 'limma'
The following object is masked from 'package:BiocGenerics':
plotMA
library(ggplot2)
library(BSgenome.Dmelanogaster.UCSC.dm6)
Loading required package: BSgenome
Loading required package: Biostrings
Loading required package: XVector
Attaching package: 'Biostrings'
The following object is masked from 'package:base':
strsplit
Loading required package: BiocIO
Loading required package: rtracklayer
Attaching package: 'rtracklayer'
The following object is masked from 'package:BiocIO':
FileForFormat
gatc_regions <- getGatcRegions(BSgenome.Dmelanogaster.UCSC.dm6::BSgenome.Dmelanogaster.UCSC.dm6)$regions
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in GenomeInfoDb::renameSeqlevels(x = df_, value = newStyle): invalid
seqlevels 'chrM' ignored
damsel_counts <- rbind(readRDS("../data/damsel_counts_a.rds"), readRDS("../data/damsel_counts_b.rds"))
head(damsel_counts)
Position seqnames start end width strand dam_1_SRR7948872.BAM
chr2L-82 chr2L-82 chr2L 82 230 149 * 1.0
chr2L-231 chr2L-231 chr2L 231 371 141 * 1.5
chr2L-372 chr2L-372 chr2L 372 539 168 * 2.5
chr2L-540 chr2L-540 chr2L 540 688 149 * 2.0
chr2L-689 chr2L-689 chr2L 689 829 141 * 0.0
chr2L-830 chr2L-830 chr2L 830 997 168 * 0.0
sd_1_SRR7948874.BAM dam_2_SRR7948876.BAM sd_2_SRR7948877.BAM
chr2L-82 0.33 0.0 0.0
chr2L-231 5.67 87.0 57.5
chr2L-372 6.17 88.0 58.5
chr2L-540 4.83 0.0 0.0
chr2L-689 0.00 0.5 0.5
chr2L-830 1.33 4.5 3.5
matrix <- as.matrix(damsel_counts[, grepl("bam", colnames(damsel_counts), ignore.case = TRUE)])
rownames(matrix) <- damsel_counts$Position
group = c("Dam", "Sd", "Dam", "Sd")
design = model.matrix(~group)
y = DGEList(matrix, group = group)
keep <- rowSums(cpm(y)>=0.5) >= 2
y = y[keep, ,keep.lib.sizes=FALSE]
y = calcNormFactors(y)
y = estimateDisp(y, robust = T, design = design)
fit = glmFit(y, design = design)
lrt = glmLRT(fit, coef=2)
de.Sd <- decideTestsDGE(lrt, lfc = 1)
Warning: 'decideTestsDGE' is deprecated.
Use 'decideTests' instead.
See help("Deprecated")
lrt$table$significant <- de.Sd
vissers_dm <- data.frame(lrt$table)
write.table(vissers_dm, file='../output/lrt_sd.txt', quote=F)
write.table(keep, file='../output/keep', quote=F, col.names = FALSE)
vissers_peaks <- system2("python3", args=c("../code/call_peaks.py",
"../output/keep", "../output/lrt_sd.txt", ">",
"../output/vissers_peaks.txt"))
vissers_peaks <- read.table("../output/vissers_peaks.txt")
#vissers_peaks <- readRDS("../output/vissers_peaks.rds")
names(vissers_peaks) <- c('chr', 's', 'e', "tags", 'pen', 'aveLogFC', 'sig')
vissers_peaks <- vissers_peaks %>% mutate(big = ifelse(tags > 2 | pen == 1, TRUE, FALSE))
head(vissers_peaks)
chr s e tags pen aveLogFC sig big
1 17450 17450 21359 6 1.000000 3.888205 5 TRUE
2 66423 66423 67818 2 1.000000 2.546664 2 TRUE
3 71991 71991 76558 4 1.000000 2.635863 4 TRUE
4 93378 93378 95515 4 1.000000 2.727970 4 TRUE
5 95762 95762 96492 2 0.666667 2.039309 2 FALSE
6 98317 98317 99667 4 1.000000 1.765455 2 TRUE
names(vissers_peaks) <- c('seqnames', 'start', 'end', "tags", 'pen', 'aveLogFC', 'sig', "big")
vissers_peaks
seqnames start end tags pen aveLogFC sig big
1 17450 17450 21359 6 1.000000 3.888205 5 TRUE
2 66423 66423 67818 2 1.000000 2.546664 2 TRUE
3 71991 71991 76558 4 1.000000 2.635863 4 TRUE
4 93378 93378 95515 4 1.000000 2.727970 4 TRUE
5 95762 95762 96492 2 0.666667 2.039309 2 FALSE
6 98317 98317 99667 4 1.000000 1.765455 2 TRUE
7 128082 128082 129234 2 1.000000 2.764402 2 TRUE
8 153859 153859 159412 14 1.000000 3.293462 14 TRUE
9 162541 162541 165173 2 1.000000 2.203177 2 TRUE
10 174384 174384 180712 10 1.000000 2.707416 7 TRUE
11 184870 184870 185704 2 1.000000 2.170039 2 TRUE
12 218515 218515 223187 4 1.000000 2.657696 3 TRUE
13 244817 244817 246651 2 1.000000 2.581034 2 TRUE
14 284481 284481 287194 4 1.000000 1.780104 3 TRUE
15 341610 341610 342612 2 1.000000 1.495494 0 TRUE
16 412876 412876 413635 2 1.000000 2.454228 1 TRUE
17 415203 415203 416303 2 1.000000 1.423234 0 TRUE
18 429799 429799 431968 8 1.000000 4.186476 8 TRUE
19 454248 454248 456138 2 1.000000 1.853537 1 TRUE
20 548926 548926 550158 2 1.000000 2.193769 1 TRUE
21 827215 827215 827854 2 1.000000 1.561627 0 TRUE
22 839897 839897 840684 2 1.000000 2.414131 1 TRUE
23 849931 849931 850538 2 1.000000 2.590091 2 TRUE
24 865263 865263 866255 2 1.000000 2.304831 2 TRUE
25 961779 961779 963822 2 1.000000 2.361434 2 TRUE
26 971691 971691 975671 2 1.000000 2.587682 2 TRUE
27 1005638 1005638 1007530 4 1.000000 1.616377 2 TRUE
28 1054278 1054278 1056004 6 1.000000 2.889476 5 TRUE
29 1058318 1058318 1059963 2 1.000000 1.656522 0 TRUE
30 1062042 1062042 1063912 4 1.000000 2.606955 4 TRUE
31 1066997 1066997 1069346 4 1.000000 1.465648 0 TRUE
32 1235573 1235573 1236711 2 1.000000 1.689239 1 TRUE
33 1243874 1243874 1248457 4 1.000000 2.091626 3 TRUE
34 1250770 1250770 1257250 10 1.000000 2.805777 10 TRUE
35 1272898 1272898 1274225 2 1.000000 1.447579 1 TRUE
36 1389584 1389584 1391298 2 1.000000 2.185734 2 TRUE
37 1394397 1394397 1397198 4 1.000000 2.159060 2 TRUE
38 1403987 1403987 1405382 4 1.000000 2.046401 2 TRUE
39 1419401 1419401 1420669 4 1.000000 2.534797 4 TRUE
40 1427775 1427775 1431909 6 1.000000 1.771697 3 TRUE
41 1434265 1434265 1441334 14 1.000000 1.884728 11 TRUE
42 1460766 1460766 1462063 4 1.000000 2.214585 2 TRUE
43 1465660 1465660 1470035 8 1.000000 2.062357 8 TRUE
44 1471893 1471893 1476640 6 1.000000 2.122235 5 TRUE
45 1644525 1644525 1651734 8 1.000000 2.345860 6 TRUE
46 1667630 1667630 1671071 10 1.000000 2.829469 10 TRUE
47 1687120 1687120 1688249 2 1.000000 1.677102 2 TRUE
48 1727558 1727558 1728462 2 1.000000 2.137160 2 TRUE
49 1885491 1885491 1886435 2 1.000000 1.927100 1 TRUE
50 1947546 1947546 1950512 6 1.000000 2.619867 6 TRUE
51 1953178 1953178 1954375 2 1.000000 2.329794 1 TRUE
52 1961005 1961005 1974362 24 1.000000 2.618727 23 TRUE
53 2161261 2161261 2162272 4 1.000000 1.949671 3 TRUE
54 2195521 2195521 2197046 2 1.000000 2.674178 2 TRUE
55 2219916 2219916 2222294 2 1.000000 1.531863 1 TRUE
56 2362836 2362836 2365681 4 1.000000 2.105246 4 TRUE
57 2381318 2381318 2383495 6 1.000000 2.685224 5 TRUE
58 2386215 2386215 2394503 10 1.000000 2.139270 8 TRUE
59 2576191 2576191 2578197 6 1.000000 3.632408 6 TRUE
60 2636132 2636132 2639439 4 1.000000 1.958060 4 TRUE
61 2692975 2692975 2695855 4 1.000000 2.401805 3 TRUE
62 2750205 2750205 2752616 4 1.000000 2.392874 4 TRUE
63 2752701 2752701 2756864 6 1.000000 2.479492 5 TRUE
64 2765376 2765376 2766752 4 1.000000 2.804281 4 TRUE
65 2769487 2769487 2770564 4 1.000000 3.327058 4 TRUE
66 2884472 2884472 2888751 6 1.000000 2.575758 6 TRUE
67 2891230 2891230 2898982 8 1.000000 2.522635 7 TRUE
68 2999302 2999302 3000289 2 1.000000 2.102168 2 TRUE
69 3038696 3038696 3039501 2 1.000000 2.205700 1 TRUE
70 3151946 3151946 3153338 2 1.000000 1.710466 2 TRUE
71 3423607 3423607 3424972 2 1.000000 1.984895 2 TRUE
72 3449917 3449917 3450445 2 1.000000 2.853132 2 TRUE
73 3602046 3602046 3603396 2 1.000000 2.288242 2 TRUE
74 3655415 3655415 3659779 8 1.000000 2.944126 8 TRUE
75 3672454 3672454 3674997 4 1.000000 1.870747 3 TRUE
76 3681376 3681376 3685945 4 1.000000 1.839272 3 TRUE
77 3712345 3712345 3712889 2 1.000000 2.474752 2 TRUE
78 3804748 3804748 3807065 4 1.000000 3.359631 4 TRUE
79 3815895 3815895 3819393 4 1.000000 2.517560 3 TRUE
80 3825187 3825187 3826062 2 1.000000 1.581810 0 TRUE
81 3839670 3839670 3842232 4 1.000000 2.079557 3 TRUE
82 3843881 3843881 3845318 2 1.000000 1.960554 2 TRUE
83 3871296 3871296 3872026 2 0.500000 2.433507 1 FALSE
84 3973642 3973642 3982252 6 1.000000 1.897895 5 TRUE
85 4119094 4119094 4120962 4 1.000000 2.074174 4 TRUE
86 4130598 4130598 4133253 8 1.000000 2.632599 7 TRUE
87 4255166 4255166 4255678 2 1.000000 1.548697 1 TRUE
88 4325015 4325015 4326580 2 1.000000 2.269388 1 TRUE
89 4334021 4334021 4336317 2 1.000000 2.248263 2 TRUE
90 4348071 4348071 4352902 10 1.000000 2.091327 8 TRUE
91 4355539 4355539 4357806 4 1.000000 2.005192 4 TRUE
92 4370298 4370298 4374513 6 1.000000 2.059106 4 TRUE
93 4381847 4381847 4382276 2 1.000000 1.857429 1 TRUE
94 4810290 4810290 4811557 2 1.000000 2.185987 2 TRUE
95 4885440 4885440 4886016 2 0.666667 2.084708 2 FALSE
96 5010524 5010524 5011505 2 1.000000 2.174446 1 TRUE
97 5018394 5018394 5020382 2 1.000000 1.542248 0 TRUE
98 5037416 5037416 5038658 2 1.000000 1.774152 2 TRUE
99 5052215 5052215 5053454 2 1.000000 2.064249 1 TRUE
100 5095885 5095885 5097126 2 1.000000 1.982954 2 TRUE
101 5239343 5239343 5240643 2 1.000000 2.002171 1 TRUE
102 5287657 5287657 5288610 2 1.000000 2.661487 1 TRUE
103 5290015 5290015 5294292 6 1.000000 3.763164 5 TRUE
104 5305796 5305796 5307437 2 1.000000 3.011279 2 TRUE
105 5429900 5429900 5434209 6 1.000000 1.852624 5 TRUE
106 5752480 5752480 5754369 2 1.000000 1.617146 0 TRUE
107 5975611 5975611 5981057 10 1.000000 2.325676 9 TRUE
108 5997097 5997097 5999119 4 1.000000 2.283502 4 TRUE
109 6000890 6000890 6001967 2 1.000000 2.002348 0 TRUE
110 6036807 6036807 6038385 4 1.000000 3.582710 4 TRUE
111 6076605 6076605 6082732 12 1.000000 2.634917 12 TRUE
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113 6103500 6103500 6108864 8 1.000000 2.036111 6 TRUE
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115 6182658 6182658 6185866 8 1.000000 2.127340 6 TRUE
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117 6208427 6208427 6211591 2 1.000000 1.443512 0 TRUE
118 6213415 6213415 6215417 4 1.000000 2.207298 3 TRUE
119 6233326 6233326 6243603 12 1.000000 2.173921 8 TRUE
120 6314409 6314409 6319003 2 1.000000 1.707554 2 TRUE
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122 6539534 6539534 6541146 4 1.000000 2.291928 4 TRUE
123 6543981 6543981 6545186 2 1.000000 2.077318 2 TRUE
124 6546436 6546436 6550700 10 1.000000 2.455224 9 TRUE
125 6611520 6611520 6612549 2 1.000000 3.444271 2 TRUE
126 6786848 6786848 6788926 2 1.000000 1.562559 0 TRUE
127 6791754 6791754 6793242 4 1.000000 2.699613 3 TRUE
128 6794965 6794965 6796037 2 1.000000 2.044484 2 TRUE
129 6813625 6813625 6815312 2 1.000000 1.383645 0 TRUE
130 6841747 6841747 6843314 2 1.000000 1.892039 2 TRUE
131 6852190 6852190 6853612 2 1.000000 3.317773 2 TRUE
132 6912799 6912799 6914662 2 1.000000 3.096580 2 TRUE
133 6918137 6918137 6922498 2 1.000000 2.842440 2 TRUE
134 6955810 6955810 6956360 2 1.000000 2.647441 1 TRUE
135 6966742 6966742 6968742 2 1.000000 2.580333 2 TRUE
136 7022782 7022782 7023861 4 1.000000 2.295130 3 TRUE
137 7026017 7026017 7028278 4 1.000000 2.556502 3 TRUE
138 7042276 7042276 7043499 2 1.000000 3.502899 1 TRUE
139 7265635 7265635 7266502 2 1.000000 1.826932 1 TRUE
140 7268935 7268935 7273324 10 1.000000 2.881090 10 TRUE
141 7387415 7387415 7389879 8 1.000000 3.476423 7 TRUE
142 7421899 7421899 7427291 10 1.000000 2.521017 8 TRUE
143 7480235 7480235 7480871 2 0.666667 1.772295 0 FALSE
144 7494525 7494525 7495639 2 1.000000 3.235146 2 TRUE
145 7542976 7542976 7544250 4 1.000000 2.667079 3 TRUE
146 7591033 7591033 7593785 8 1.000000 3.146072 7 TRUE
147 7636122 7636122 7639068 8 1.000000 2.081855 5 TRUE
148 7809097 7809097 7810606 2 1.000000 3.136458 2 TRUE
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151 7991781 7991781 7994123 4 1.000000 2.189142 3 TRUE
152 8030157 8030157 8030447 2 1.000000 3.620322 2 TRUE
153 8081135 8081135 8086848 4 1.000000 3.108266 4 TRUE
154 8211272 8211272 8212523 2 1.000000 2.346605 2 TRUE
155 8212948 8212948 8214626 6 1.000000 1.994204 4 TRUE
156 8300916 8300916 8304980 12 1.000000 3.473635 11 TRUE
157 8363913 8363913 8367727 6 1.000000 3.329240 6 TRUE
158 8382543 8382543 8383531 4 1.000000 2.145963 3 TRUE
159 8403289 8403289 8404030 2 0.666667 4.278901 2 FALSE
160 8414615 8414615 8417868 8 1.000000 2.680360 7 TRUE
161 8422765 8422765 8426730 6 1.000000 2.990551 6 TRUE
162 8436914 8436914 8438221 6 1.000000 2.927576 6 TRUE
163 8515380 8515380 8516045 2 1.000000 2.040472 2 TRUE
164 8516985 8516985 8517822 2 1.000000 3.228556 2 TRUE
165 8554227 8554227 8557794 6 1.000000 2.256082 5 TRUE
166 8582155 8582155 8585362 4 1.000000 2.051612 3 TRUE
167 8603568 8603568 8606117 4 1.000000 1.947372 3 TRUE
168 8611431 8611431 8614493 6 1.000000 1.636655 4 TRUE
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1899 1230456 1230456 1234693 6 1.000000 2.866410 4 TRUE
1900 1253821 1253821 1254204 2 1.000000 2.070888 2 TRUE
1901 1379369 1379369 1380637 2 1.000000 2.111553 1 TRUE
1902 1523487 1523487 1526892 4 1.000000 1.849164 3 TRUE
1903 1548592 1548592 1551541 4 1.000000 1.626573 1 TRUE
1904 1566015 1566015 1566654 2 1.000000 2.265209 2 TRUE
1905 1593102 1593102 1593778 2 0.500000 3.380290 1 FALSE
1906 1612338 1612338 1613563 4 1.000000 1.889304 3 TRUE
1907 1616289 1616289 1618665 8 1.000000 2.345085 7 TRUE
1908 1621569 1621569 1622069 2 1.000000 3.237541 2 TRUE
1909 1669702 1669702 1670890 2 1.000000 3.197225 2 TRUE
1910 1691306 1691306 1693221 4 1.000000 3.053160 4 TRUE
1911 1756674 1756674 1757672 2 1.000000 1.516305 0 TRUE
1912 1814521 1814521 1816013 2 1.000000 1.818760 1 TRUE
1913 1865729 1865729 1866383 2 0.666667 1.557356 1 FALSE
1914 1896723 1896723 1899899 4 1.000000 3.139443 4 TRUE
1915 1908025 1908025 1909594 4 1.000000 3.049109 4 TRUE
1916 1941209 1941209 1946286 12 1.000000 2.723163 12 TRUE
1917 1948494 1948494 1950184 2 1.000000 1.674414 1 TRUE
1918 2009251 2009251 2010948 2 1.000000 2.608314 1 TRUE
1919 2061775 2061775 2062492 2 1.000000 1.634321 1 TRUE
1920 2080232 2080232 2083557 10 1.000000 2.570532 9 TRUE
1921 2085195 2085195 2087126 2 1.000000 1.935374 1 TRUE
1922 2097226 2097226 2097812 2 0.666667 2.229319 0 FALSE
1923 2123133 2123133 2123977 2 1.000000 2.652978 2 TRUE
1924 2134095 2134095 2141278 14 1.000000 2.250206 12 TRUE
1925 2295137 2295137 2302647 6 1.000000 1.991647 5 TRUE
1926 2306622 2306622 2308676 2 1.000000 2.417980 1 TRUE
1927 2316971 2316971 2326041 14 1.000000 2.247572 13 TRUE
1928 2330287 2330287 2332070 2 1.000000 1.638238 1 TRUE
1929 2352148 2352148 2353173 4 1.000000 2.714585 3 TRUE
1930 2362017 2362017 2363843 4 1.000000 2.272911 3 TRUE
1931 2387516 2387516 2388139 2 0.285714 3.735662 2 FALSE
1932 2388816 2388816 2389278 2 0.500000 2.427697 1 FALSE
1933 2394949 2394949 2399401 12 1.000000 3.127936 12 TRUE
1934 2410411 2410411 2411863 4 1.000000 2.254572 3 TRUE
1935 2417487 2417487 2422192 8 1.000000 2.514492 8 TRUE
1936 2603528 2603528 2604631 6 1.000000 3.228324 6 TRUE
1937 2635160 2635160 2637605 4 1.000000 3.186378 4 TRUE
1938 2640229 2640229 2644703 6 1.000000 2.383969 5 TRUE
1939 2764490 2764490 2766457 2 1.000000 2.801657 2 TRUE
1940 2787483 2787483 2788543 2 1.000000 2.584479 2 TRUE
1941 2796199 2796199 2796689 2 1.000000 2.219572 2 TRUE
1942 2987472 2987472 2988305 2 1.000000 2.674065 2 TRUE
1943 3004139 3004139 3004848 2 1.000000 2.411284 2 TRUE
1944 3133925 3133925 3136177 4 1.000000 2.725395 3 TRUE
1945 3137252 3137252 3139350 8 1.000000 3.329492 8 TRUE
1946 3158958 3158958 3162199 8 1.000000 2.856090 7 TRUE
1947 3175374 3175374 3176838 4 1.000000 2.096810 2 TRUE
1948 3368040 3368040 3373881 4 1.000000 2.435317 3 TRUE
1949 3375847 3375847 3382043 6 1.000000 2.248055 6 TRUE
1950 3428372 3428372 3430081 4 1.000000 2.605265 4 TRUE
1951 3449348 3449348 3451859 4 1.000000 2.072431 4 TRUE
1952 3485229 3485229 3486491 6 1.000000 3.296105 6 TRUE
1953 3530657 3530657 3531688 2 1.000000 3.052687 2 TRUE
1954 3542270 3542270 3545762 4 1.000000 2.319234 4 TRUE
1955 3572187 3572187 3573602 2 1.000000 2.083327 1 TRUE
1956 3673487 3673487 3687931 14 1.000000 2.339216 11 TRUE
1957 3692272 3692272 3695235 2 1.000000 2.238370 1 TRUE
1958 3702843 3702843 3704579 2 1.000000 2.932530 2 TRUE
1959 3718194 3718194 3721581 8 1.000000 2.682280 7 TRUE
1960 3748070 3748070 3752656 2 1.000000 2.249401 2 TRUE
1961 3768483 3768483 3773456 2 1.000000 1.553173 2 TRUE
1962 3796501 3796501 3799214 8 1.000000 2.967217 8 TRUE
1963 3949670 3949670 3950842 2 1.000000 1.693296 1 TRUE
1964 3971962 3971962 3973342 2 1.000000 1.698422 1 TRUE
1965 4020169 4020169 4021044 2 1.000000 2.376289 2 TRUE
1966 4087584 4087584 4087953 2 0.500000 3.428208 2 FALSE
1967 4193094 4193094 4194316 2 1.000000 1.730172 1 TRUE
1968 4206878 4206878 4211915 6 1.000000 2.019723 4 TRUE
1969 4280692 4280692 4283094 2 1.000000 2.482962 1 TRUE
1970 4395532 4395532 4396498 2 1.000000 1.757026 2 TRUE
1971 4405613 4405613 4406876 2 1.000000 1.857251 1 TRUE
1972 4411442 4411442 4413884 2 1.000000 1.671995 0 TRUE
1973 4415245 4415245 4416254 2 1.000000 2.044880 1 TRUE
1974 4508232 4508232 4519769 10 1.000000 2.090374 9 TRUE
1975 4646523 4646523 4647339 2 1.000000 3.315185 2 TRUE
1976 4648902 4648902 4649689 2 0.666667 3.995354 2 FALSE
1977 4685186 4685186 4695884 10 1.000000 2.994920 9 TRUE
1978 5139673 5139673 5143682 4 1.000000 2.085187 3 TRUE
1979 5417374 5417374 5423029 8 1.000000 3.098140 7 TRUE
1980 5562088 5562088 5564910 2 1.000000 2.648370 2 TRUE
1981 5567747 5567747 5569753 4 1.000000 1.502560 1 TRUE
1982 5576958 5576958 5580293 6 1.000000 1.612370 2 TRUE
1983 5597587 5597587 5603862 4 1.000000 1.552907 1 TRUE
1984 5620988 5620988 5622374 4 1.000000 3.308471 4 TRUE
1985 5636445 5636445 5641685 6 1.000000 2.373504 6 TRUE
1986 5644473 5644473 5645556 2 1.000000 1.306303 0 TRUE
1987 5645997 5645997 5647187 4 1.000000 2.559969 4 TRUE
1988 5661697 5661697 5664964 4 1.000000 3.075767 4 TRUE
1989 5677609 5677609 5678791 4 1.000000 4.033083 4 TRUE
1990 5690089 5690089 5691308 4 1.000000 2.158686 2 TRUE
1991 5746106 5746106 5747521 2 1.000000 2.664221 2 TRUE
1992 5884115 5884115 5889196 12 1.000000 2.884591 12 TRUE
1993 5893814 5893814 5897951 6 1.000000 2.037956 4 TRUE
1994 5900207 5900207 5902416 4 1.000000 2.839875 4 TRUE
1995 5995653 5995653 5996783 2 1.000000 2.236852 2 TRUE
1996 6030443 6030443 6032841 6 1.000000 2.611494 6 TRUE
1997 6278433 6278433 6280767 6 1.000000 2.968160 2 TRUE
1998 6281739 6281739 6282412 2 1.000000 3.873618 2 TRUE
1999 6282721 6282721 6285016 6 1.000000 2.968494 6 TRUE
2000 6291836 6291836 6293538 4 1.000000 2.295145 3 TRUE
2001 6334215 6334215 6338195 6 1.000000 2.026189 5 TRUE
2002 6599283 6599283 6601558 4 1.000000 2.460159 3 TRUE
2003 6661944 6661944 6663747 4 1.000000 2.015484 3 TRUE
2004 6694341 6694341 6695681 2 1.000000 2.529447 2 TRUE
2005 6706285 6706285 6708191 2 1.000000 3.518919 2 TRUE
2006 6803417 6803417 6806779 12 1.000000 2.186025 10 TRUE
2007 6854438 6854438 6861966 10 1.000000 2.320646 8 TRUE
2008 6869018 6869018 6870587 4 1.000000 1.798679 2 TRUE
2009 6873392 6873392 6874836 2 1.000000 1.794778 0 TRUE
2010 6978281 6978281 6982912 8 1.000000 2.056908 7 TRUE
2011 6985154 6985154 6990074 12 1.000000 3.974858 12 TRUE
2012 6997050 6997050 6999034 4 1.000000 3.114436 4 TRUE
2013 7095467 7095467 7097637 6 1.000000 3.355950 6 TRUE
2014 7118585 7118585 7123823 6 1.000000 2.049829 3 TRUE
2015 7138091 7138091 7139835 2 1.000000 1.703422 0 TRUE
2016 7147461 7147461 7155264 10 1.000000 2.260718 9 TRUE
2017 7163618 7163618 7167769 10 1.000000 2.961378 10 TRUE
2018 7198945 7198945 7201876 2 1.000000 1.533284 1 TRUE
2019 7223538 7223538 7225754 4 1.000000 2.028722 2 TRUE
2020 7232533 7232533 7234455 4 1.000000 1.808122 4 TRUE
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2024 7304959 7304959 7308100 4 1.000000 1.860798 2 TRUE
2025 7317974 7317974 7319013 4 1.000000 2.516386 3 TRUE
2026 7324365 7324365 7325254 2 1.000000 2.204043 2 TRUE
2027 7355466 7355466 7356100 2 1.000000 1.357450 0 TRUE
2028 7459136 7459136 7463293 6 1.000000 2.197181 5 TRUE
2029 7596017 7596017 7596967 4 1.000000 2.305773 2 TRUE
2030 7599372 7599372 7601937 4 1.000000 2.210050 4 TRUE
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2032 7639961 7639961 7642449 2 1.000000 2.216766 2 TRUE
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2034 7696271 7696271 7701942 2 1.000000 2.053748 1 TRUE
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2039 7935725 7935725 7937064 2 1.000000 2.049861 1 TRUE
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2235 19259908 19259908 19261172 4 1.000000 2.092459 3 TRUE
2236 19353639 19353639 19354676 2 1.000000 1.622793 2 TRUE
2237 19371153 19371153 19371826 2 1.000000 2.743750 2 TRUE
2238 19423139 19423139 19426351 2 1.000000 2.600025 2 TRUE
2239 19430774 19430774 19434556 4 1.000000 1.963896 3 TRUE
2240 19469069 19469069 19469568 2 0.666667 2.044365 1 FALSE
2241 19495598 19495598 19497639 2 1.000000 2.431401 2 TRUE
2242 19508668 19508668 19512283 6 1.000000 1.615169 2 TRUE
2243 19520740 19520740 19521451 2 1.000000 2.304879 2 TRUE
2244 19537121 19537121 19538345 2 1.000000 2.150287 2 TRUE
2245 19555039 19555039 19558855 4 1.000000 2.889909 4 TRUE
2246 19577604 19577604 19579155 2 1.000000 3.024873 1 TRUE
2247 19692928 19692928 19694717 2 1.000000 2.247196 1 TRUE
2248 19717068 19717068 19720972 4 1.000000 1.951301 3 TRUE
2249 19730658 19730658 19731512 2 1.000000 3.223368 2 TRUE
2250 19734431 19734431 19736390 6 1.000000 2.688969 6 TRUE
2251 19743787 19743787 19744204 2 0.666667 2.511750 1 FALSE
2252 19779210 19779210 19780527 2 1.000000 1.769486 1 TRUE
2253 19782810 19782810 19784209 6 1.000000 2.617602 6 TRUE
2254 19785134 19785134 19786535 2 1.000000 1.593646 1 TRUE
2255 19788052 19788052 19788673 2 1.000000 3.533853 2 TRUE
2256 19825330 19825330 19828733 2 1.000000 1.876801 2 TRUE
2257 19842245 19842245 19843731 2 1.000000 2.107969 1 TRUE
2258 19845988 19845988 19851147 8 1.000000 3.017929 8 TRUE
2259 19878066 19878066 19879810 2 1.000000 2.935824 2 TRUE
2260 19888189 19888189 19888663 2 1.000000 2.803774 2 TRUE
2261 19889632 19889632 19890955 2 1.000000 2.444430 2 TRUE
2262 19910596 19910596 19912261 4 1.000000 2.565299 4 TRUE
2263 19916924 19916924 19917537 2 1.000000 2.337820 2 TRUE
2264 20109252 20109252 20109781 2 1.000000 2.057673 1 TRUE
2265 20498974 20498974 20502479 4 1.000000 2.405451 4 TRUE
2266 20552663 20552663 20553674 2 1.000000 1.933296 1 TRUE
2267 20642930 20642930 20646183 10 1.000000 1.622488 2 TRUE
2268 20664622 20664622 20668465 6 1.000000 1.995289 5 TRUE
2269 20717432 20717432 20718035 2 1.000000 1.623155 1 TRUE
2270 20724066 20724066 20726335 6 1.000000 2.571329 6 TRUE
2271 20728397 20728397 20731120 2 1.000000 1.664190 1 TRUE
2272 20747515 20747515 20748921 2 1.000000 2.290085 2 TRUE
2273 20751677 20751677 20752508 2 1.000000 1.663622 1 TRUE
2274 20754775 20754775 20755982 4 1.000000 1.741615 3 TRUE
2275 21052821 21052821 21055674 4 1.000000 2.802712 3 TRUE
2276 21281511 21281511 21282783 2 1.000000 2.070810 2 TRUE
2277 21367097 21367097 21369529 2 1.000000 2.593883 2 TRUE
2278 21598172 21598172 21598796 2 1.000000 1.581359 1 TRUE
2279 22068565 22068565 22070912 2 1.000000 1.821728 1 TRUE
2280 22221706 22221706 22222296 2 1.000000 2.278239 2 TRUE
2281 22454706 22454706 22456974 2 1.000000 3.408969 2 TRUE
2282 22479396 22479396 22481979 4 1.000000 1.958655 3 TRUE
2283 22832217 22832217 22832694 2 1.000000 1.284323 0 TRUE
2284 23401220 23401220 23402427 2 1.000000 2.047473 2 TRUE
2285 1379039 1379039 1380649 2 1.000000 2.002104 1 TRUE
2286 1793673 1793673 1795678 2 1.000000 2.878117 2 TRUE
chromosome names are missing
add manually - assuming that chromosomes remain in order - 2L, 2R, 3L, 3R, 4, etc
first break apart the peaks - the peaks are organised by increasing start site
so if the start site is less than the previous peak - new chromosome
how many peaks per chromosome?
peaks_per_chr <- vissers_peaks %>%
mutate(end_chr = ifelse(start > lead(start), TRUE, NA),
row_num = 1:nrow(.),
end_chr = ifelse(row_num == nrow(.), TRUE, end_chr),
end_pos = ifelse(end_chr == TRUE, row_num, NA)) %>%
fill(end_pos, .direction = "up") %>%
distinct(end_pos) %>%
mutate(num_per_chr = end_pos - lag(end_pos),
num_per_chr = ifelse(is.na(num_per_chr), end_pos, num_per_chr)) %>%
.$num_per_chr
peaks_per_chr
[1] 390 421 478 544 39 412 2
chromosomes <- sub("-.*", "", rownames(vissers_dm)) %>% unique()
chromosomes
[1] "chr2L" "chr2R" "chr3L" "chr3R" "chr4" "chrX" "chrY"
length(chromosomes) == length(peaks_per_chr)
[1] TRUE
chr_vec <- character()
for(i in 1:length(peaks_per_chr)) {
chr_vec <- c(chr_vec, rep(chromosomes[i], times = peaks_per_chr[i]))
}
vissers_peaks$seqnames <- chr_vec
head(vissers_peaks)
seqnames start end tags pen aveLogFC sig big
1 chr2L 17450 21359 6 1.000000 3.888205 5 TRUE
2 chr2L 66423 67818 2 1.000000 2.546664 2 TRUE
3 chr2L 71991 76558 4 1.000000 2.635863 4 TRUE
4 chr2L 93378 95515 4 1.000000 2.727970 4 TRUE
5 chr2L 95762 96492 2 0.666667 2.039309 2 FALSE
6 chr2L 98317 99667 4 1.000000 1.765455 2 TRUE
sessionInfo()
R Under development (unstable) (2024-01-17 r85813)
Platform: x86_64-apple-darwin20
Running under: macOS Sonoma 14.1.1
Matrix products: default
BLAS: /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRlapack.dylib; LAPACK version 3.12.0
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
time zone: Australia/Melbourne
tzcode source: internal
attached base packages:
[1] stats4 stats graphics grDevices datasets utils methods
[8] base
other attached packages:
[1] BSgenome.Dmelanogaster.UCSC.dm6_1.4.1 BSgenome_1.72.0
[3] rtracklayer_1.64.0 BiocIO_1.14.0
[5] Biostrings_2.72.1 XVector_0.44.0
[7] ggplot2_3.5.1 edgeR_4.2.1
[9] limma_3.60.4 tidyr_1.3.1
[11] dplyr_1.1.4 plyranges_1.24.0
[13] GenomicRanges_1.56.1 GenomeInfoDb_1.40.1
[15] IRanges_2.38.1 S4Vectors_0.42.1
[17] BiocGenerics_0.50.0 Damsel_1.0.0
[19] workflowr_1.7.1
loaded via a namespace (and not attached):
[1] tidyselect_1.2.1 bitops_1.0-8
[3] fastmap_1.2.0 RCurl_1.98-1.16
[5] GenomicAlignments_1.40.0 promises_1.3.0
[7] XML_3.99-0.17 digest_0.6.35
[9] lifecycle_1.0.4 statmod_1.5.0
[11] processx_3.8.4 magrittr_2.0.3.9000
[13] compiler_4.4.0 rlang_1.1.3
[15] sass_0.4.9 tools_4.4.0
[17] utf8_1.2.4 yaml_2.3.8
[19] knitr_1.46 S4Arrays_1.4.1
[21] curl_5.2.1 DelayedArray_0.30.1
[23] abind_1.4-5 BiocParallel_1.38.0
[25] withr_3.0.1 purrr_1.0.2
[27] grid_4.4.0 fansi_1.0.6
[29] git2r_0.33.0 colorspace_2.1-1
[31] scales_1.3.0 SummarizedExperiment_1.34.0
[33] cli_3.6.2 rmarkdown_2.27
[35] crayon_1.5.3 generics_0.1.3
[37] rstudioapi_0.16.0 httr_1.4.7
[39] rjson_0.2.21 cachem_1.1.0
[41] stringr_1.5.1.9000 splines_4.4.0
[43] zlibbioc_1.50.0 parallel_4.4.0
[45] BiocManager_1.30.23 restfulr_0.0.15
[47] matrixStats_1.3.0 vctrs_0.6.5
[49] Matrix_1.7-0 jsonlite_1.8.8
[51] callr_3.7.6 locfit_1.5-9.10
[53] jquerylib_0.1.4 glue_1.7.0
[55] codetools_0.2-20 ps_1.7.6
[57] stringi_1.8.4 gtable_0.3.5
[59] later_1.3.2 UCSC.utils_1.0.0
[61] munsell_0.5.1 tibble_3.2.1
[63] pillar_1.9.0 htmltools_0.5.8.1
[65] GenomeInfoDbData_1.2.12 R6_2.5.1
[67] rprojroot_2.0.4 evaluate_0.23
[69] Biobase_2.64.0 lattice_0.22-6
[71] Rsamtools_2.20.0 renv_1.0.7
[73] httpuv_1.6.15 bslib_0.7.0
[75] Rcpp_1.0.12 SparseArray_1.4.8
[77] whisker_0.4.1 xfun_0.44
[79] fs_1.6.4 MatrixGenerics_1.16.0
[81] getPass_0.2-4 pkgconfig_2.0.3