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Knit directory: damsel_paper/analysis/

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Introduction

library(Damsel)
library(plyranges)
Loading required package: BiocGenerics

Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':

    anyDuplicated, aperm, append, as.data.frame, basename, cbind,
    colnames, dirname, do.call, duplicated, eval, evalq, Filter, Find,
    get, grep, grepl, intersect, is.unsorted, lapply, Map, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    Position, rank, rbind, Reduce, rownames, sapply, setdiff, table,
    tapply, union, unique, unsplit, which.max, which.min
Loading required package: IRanges
Warning: package 'IRanges' was built under R version 4.4.1
Loading required package: S4Vectors
Warning: package 'S4Vectors' was built under R version 4.4.1
Loading required package: stats4

Attaching package: 'S4Vectors'
The following object is masked from 'package:utils':

    findMatches
The following objects are masked from 'package:base':

    expand.grid, I, unname
Loading required package: GenomicRanges
Loading required package: GenomeInfoDb

Attaching package: 'plyranges'
The following object is masked from 'package:IRanges':

    slice
The following object is masked from 'package:stats':

    filter
library(dplyr)

Attaching package: 'dplyr'
The following objects are masked from 'package:plyranges':

    between, n, n_distinct
The following objects are masked from 'package:GenomicRanges':

    intersect, setdiff, union
The following object is masked from 'package:GenomeInfoDb':

    intersect
The following objects are masked from 'package:IRanges':

    collapse, desc, intersect, setdiff, slice, union
The following objects are masked from 'package:S4Vectors':

    first, intersect, rename, setdiff, setequal, union
The following objects are masked from 'package:BiocGenerics':

    combine, intersect, setdiff, union
The following objects are masked from 'package:stats':

    filter, lag
The following objects are masked from 'package:base':

    intersect, setdiff, setequal, union
library(tidyr)

Attaching package: 'tidyr'
The following object is masked from 'package:S4Vectors':

    expand
library(edgeR)
Warning: package 'edgeR' was built under R version 4.4.1
Loading required package: limma
Warning: package 'limma' was built under R version 4.4.1

Attaching package: 'limma'
The following object is masked from 'package:BiocGenerics':

    plotMA
library(ggplot2)

library(BSgenome.Dmelanogaster.UCSC.dm6)
Loading required package: BSgenome
Loading required package: Biostrings
Loading required package: XVector

Attaching package: 'Biostrings'
The following object is masked from 'package:base':

    strsplit
Loading required package: BiocIO
Loading required package: rtracklayer

Attaching package: 'rtracklayer'
The following object is masked from 'package:BiocIO':

    FileForFormat
gatc_regions <- getGatcRegions(BSgenome.Dmelanogaster.UCSC.dm6::BSgenome.Dmelanogaster.UCSC.dm6)$regions
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in .local(x, row.names, optional, ...): 'optional' argument was ignored
Warning in GenomeInfoDb::renameSeqlevels(x = df_, value = newStyle): invalid
seqlevels 'chrM' ignored
damsel_counts <- rbind(readRDS("../data/damsel_counts_a.rds"), readRDS("../data/damsel_counts_b.rds"))
head(damsel_counts)
           Position seqnames start end width strand dam_1_SRR7948872.BAM
chr2L-82   chr2L-82    chr2L    82 230   149      *                  1.0
chr2L-231 chr2L-231    chr2L   231 371   141      *                  1.5
chr2L-372 chr2L-372    chr2L   372 539   168      *                  2.5
chr2L-540 chr2L-540    chr2L   540 688   149      *                  2.0
chr2L-689 chr2L-689    chr2L   689 829   141      *                  0.0
chr2L-830 chr2L-830    chr2L   830 997   168      *                  0.0
          sd_1_SRR7948874.BAM dam_2_SRR7948876.BAM sd_2_SRR7948877.BAM
chr2L-82                 0.33                  0.0                 0.0
chr2L-231                5.67                 87.0                57.5
chr2L-372                6.17                 88.0                58.5
chr2L-540                4.83                  0.0                 0.0
chr2L-689                0.00                  0.5                 0.5
chr2L-830                1.33                  4.5                 3.5
matrix <- as.matrix(damsel_counts[, grepl("bam", colnames(damsel_counts), ignore.case = TRUE)])
rownames(matrix) <- damsel_counts$Position
group = c("Dam", "Sd", "Dam", "Sd")
design = model.matrix(~group)
y = DGEList(matrix, group = group)
keep <- rowSums(cpm(y)>=0.5) >= 2
y = y[keep, ,keep.lib.sizes=FALSE]
y = calcNormFactors(y)
y = estimateDisp(y, robust = T, design = design)

fit = glmFit(y, design = design)
lrt = glmLRT(fit, coef=2)
de.Sd <- decideTestsDGE(lrt, lfc = 1)
Warning: 'decideTestsDGE' is deprecated.
Use 'decideTests' instead.
See help("Deprecated")
lrt$table$significant <- de.Sd

vissers_dm <- data.frame(lrt$table)
write.table(vissers_dm, file='../output/lrt_sd.txt', quote=F)
write.table(keep, file='../output/keep', quote=F, col.names = FALSE)
vissers_peaks <- system2("python3", args=c("../code/call_peaks.py",
        "../output/keep", "../output/lrt_sd.txt", ">",
        "../output/vissers_peaks.txt"))
vissers_peaks <- read.table("../output/vissers_peaks.txt")
#vissers_peaks <- readRDS("../output/vissers_peaks.rds")
names(vissers_peaks) <- c('chr', 's', 'e', "tags", 'pen', 'aveLogFC', 'sig')
vissers_peaks <- vissers_peaks %>% mutate(big = ifelse(tags > 2 | pen == 1, TRUE, FALSE))
head(vissers_peaks)
    chr     s     e tags      pen aveLogFC sig   big
1 17450 17450 21359    6 1.000000 3.888205   5  TRUE
2 66423 66423 67818    2 1.000000 2.546664   2  TRUE
3 71991 71991 76558    4 1.000000 2.635863   4  TRUE
4 93378 93378 95515    4 1.000000 2.727970   4  TRUE
5 95762 95762 96492    2 0.666667 2.039309   2 FALSE
6 98317 98317 99667    4 1.000000 1.765455   2  TRUE

vissers peaks must be modified

names(vissers_peaks) <- c('seqnames', 'start', 'end', "tags", 'pen', 'aveLogFC', 'sig', "big")
vissers_peaks
     seqnames    start      end tags      pen aveLogFC sig   big
1       17450    17450    21359    6 1.000000 3.888205   5  TRUE
2       66423    66423    67818    2 1.000000 2.546664   2  TRUE
3       71991    71991    76558    4 1.000000 2.635863   4  TRUE
4       93378    93378    95515    4 1.000000 2.727970   4  TRUE
5       95762    95762    96492    2 0.666667 2.039309   2 FALSE
6       98317    98317    99667    4 1.000000 1.765455   2  TRUE
7      128082   128082   129234    2 1.000000 2.764402   2  TRUE
8      153859   153859   159412   14 1.000000 3.293462  14  TRUE
9      162541   162541   165173    2 1.000000 2.203177   2  TRUE
10     174384   174384   180712   10 1.000000 2.707416   7  TRUE
11     184870   184870   185704    2 1.000000 2.170039   2  TRUE
12     218515   218515   223187    4 1.000000 2.657696   3  TRUE
13     244817   244817   246651    2 1.000000 2.581034   2  TRUE
14     284481   284481   287194    4 1.000000 1.780104   3  TRUE
15     341610   341610   342612    2 1.000000 1.495494   0  TRUE
16     412876   412876   413635    2 1.000000 2.454228   1  TRUE
17     415203   415203   416303    2 1.000000 1.423234   0  TRUE
18     429799   429799   431968    8 1.000000 4.186476   8  TRUE
19     454248   454248   456138    2 1.000000 1.853537   1  TRUE
20     548926   548926   550158    2 1.000000 2.193769   1  TRUE
21     827215   827215   827854    2 1.000000 1.561627   0  TRUE
22     839897   839897   840684    2 1.000000 2.414131   1  TRUE
23     849931   849931   850538    2 1.000000 2.590091   2  TRUE
24     865263   865263   866255    2 1.000000 2.304831   2  TRUE
25     961779   961779   963822    2 1.000000 2.361434   2  TRUE
26     971691   971691   975671    2 1.000000 2.587682   2  TRUE
27    1005638  1005638  1007530    4 1.000000 1.616377   2  TRUE
28    1054278  1054278  1056004    6 1.000000 2.889476   5  TRUE
29    1058318  1058318  1059963    2 1.000000 1.656522   0  TRUE
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32    1235573  1235573  1236711    2 1.000000 1.689239   1  TRUE
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1899  1230456  1230456  1234693    6 1.000000 2.866410   4  TRUE
1900  1253821  1253821  1254204    2 1.000000 2.070888   2  TRUE
1901  1379369  1379369  1380637    2 1.000000 2.111553   1  TRUE
1902  1523487  1523487  1526892    4 1.000000 1.849164   3  TRUE
1903  1548592  1548592  1551541    4 1.000000 1.626573   1  TRUE
1904  1566015  1566015  1566654    2 1.000000 2.265209   2  TRUE
1905  1593102  1593102  1593778    2 0.500000 3.380290   1 FALSE
1906  1612338  1612338  1613563    4 1.000000 1.889304   3  TRUE
1907  1616289  1616289  1618665    8 1.000000 2.345085   7  TRUE
1908  1621569  1621569  1622069    2 1.000000 3.237541   2  TRUE
1909  1669702  1669702  1670890    2 1.000000 3.197225   2  TRUE
1910  1691306  1691306  1693221    4 1.000000 3.053160   4  TRUE
1911  1756674  1756674  1757672    2 1.000000 1.516305   0  TRUE
1912  1814521  1814521  1816013    2 1.000000 1.818760   1  TRUE
1913  1865729  1865729  1866383    2 0.666667 1.557356   1 FALSE
1914  1896723  1896723  1899899    4 1.000000 3.139443   4  TRUE
1915  1908025  1908025  1909594    4 1.000000 3.049109   4  TRUE
1916  1941209  1941209  1946286   12 1.000000 2.723163  12  TRUE
1917  1948494  1948494  1950184    2 1.000000 1.674414   1  TRUE
1918  2009251  2009251  2010948    2 1.000000 2.608314   1  TRUE
1919  2061775  2061775  2062492    2 1.000000 1.634321   1  TRUE
1920  2080232  2080232  2083557   10 1.000000 2.570532   9  TRUE
1921  2085195  2085195  2087126    2 1.000000 1.935374   1  TRUE
1922  2097226  2097226  2097812    2 0.666667 2.229319   0 FALSE
1923  2123133  2123133  2123977    2 1.000000 2.652978   2  TRUE
1924  2134095  2134095  2141278   14 1.000000 2.250206  12  TRUE
1925  2295137  2295137  2302647    6 1.000000 1.991647   5  TRUE
1926  2306622  2306622  2308676    2 1.000000 2.417980   1  TRUE
1927  2316971  2316971  2326041   14 1.000000 2.247572  13  TRUE
1928  2330287  2330287  2332070    2 1.000000 1.638238   1  TRUE
1929  2352148  2352148  2353173    4 1.000000 2.714585   3  TRUE
1930  2362017  2362017  2363843    4 1.000000 2.272911   3  TRUE
1931  2387516  2387516  2388139    2 0.285714 3.735662   2 FALSE
1932  2388816  2388816  2389278    2 0.500000 2.427697   1 FALSE
1933  2394949  2394949  2399401   12 1.000000 3.127936  12  TRUE
1934  2410411  2410411  2411863    4 1.000000 2.254572   3  TRUE
1935  2417487  2417487  2422192    8 1.000000 2.514492   8  TRUE
1936  2603528  2603528  2604631    6 1.000000 3.228324   6  TRUE
1937  2635160  2635160  2637605    4 1.000000 3.186378   4  TRUE
1938  2640229  2640229  2644703    6 1.000000 2.383969   5  TRUE
1939  2764490  2764490  2766457    2 1.000000 2.801657   2  TRUE
1940  2787483  2787483  2788543    2 1.000000 2.584479   2  TRUE
1941  2796199  2796199  2796689    2 1.000000 2.219572   2  TRUE
1942  2987472  2987472  2988305    2 1.000000 2.674065   2  TRUE
1943  3004139  3004139  3004848    2 1.000000 2.411284   2  TRUE
1944  3133925  3133925  3136177    4 1.000000 2.725395   3  TRUE
1945  3137252  3137252  3139350    8 1.000000 3.329492   8  TRUE
1946  3158958  3158958  3162199    8 1.000000 2.856090   7  TRUE
1947  3175374  3175374  3176838    4 1.000000 2.096810   2  TRUE
1948  3368040  3368040  3373881    4 1.000000 2.435317   3  TRUE
1949  3375847  3375847  3382043    6 1.000000 2.248055   6  TRUE
1950  3428372  3428372  3430081    4 1.000000 2.605265   4  TRUE
1951  3449348  3449348  3451859    4 1.000000 2.072431   4  TRUE
1952  3485229  3485229  3486491    6 1.000000 3.296105   6  TRUE
1953  3530657  3530657  3531688    2 1.000000 3.052687   2  TRUE
1954  3542270  3542270  3545762    4 1.000000 2.319234   4  TRUE
1955  3572187  3572187  3573602    2 1.000000 2.083327   1  TRUE
1956  3673487  3673487  3687931   14 1.000000 2.339216  11  TRUE
1957  3692272  3692272  3695235    2 1.000000 2.238370   1  TRUE
1958  3702843  3702843  3704579    2 1.000000 2.932530   2  TRUE
1959  3718194  3718194  3721581    8 1.000000 2.682280   7  TRUE
1960  3748070  3748070  3752656    2 1.000000 2.249401   2  TRUE
1961  3768483  3768483  3773456    2 1.000000 1.553173   2  TRUE
1962  3796501  3796501  3799214    8 1.000000 2.967217   8  TRUE
1963  3949670  3949670  3950842    2 1.000000 1.693296   1  TRUE
1964  3971962  3971962  3973342    2 1.000000 1.698422   1  TRUE
1965  4020169  4020169  4021044    2 1.000000 2.376289   2  TRUE
1966  4087584  4087584  4087953    2 0.500000 3.428208   2 FALSE
1967  4193094  4193094  4194316    2 1.000000 1.730172   1  TRUE
1968  4206878  4206878  4211915    6 1.000000 2.019723   4  TRUE
1969  4280692  4280692  4283094    2 1.000000 2.482962   1  TRUE
1970  4395532  4395532  4396498    2 1.000000 1.757026   2  TRUE
1971  4405613  4405613  4406876    2 1.000000 1.857251   1  TRUE
1972  4411442  4411442  4413884    2 1.000000 1.671995   0  TRUE
1973  4415245  4415245  4416254    2 1.000000 2.044880   1  TRUE
1974  4508232  4508232  4519769   10 1.000000 2.090374   9  TRUE
1975  4646523  4646523  4647339    2 1.000000 3.315185   2  TRUE
1976  4648902  4648902  4649689    2 0.666667 3.995354   2 FALSE
1977  4685186  4685186  4695884   10 1.000000 2.994920   9  TRUE
1978  5139673  5139673  5143682    4 1.000000 2.085187   3  TRUE
1979  5417374  5417374  5423029    8 1.000000 3.098140   7  TRUE
1980  5562088  5562088  5564910    2 1.000000 2.648370   2  TRUE
1981  5567747  5567747  5569753    4 1.000000 1.502560   1  TRUE
1982  5576958  5576958  5580293    6 1.000000 1.612370   2  TRUE
1983  5597587  5597587  5603862    4 1.000000 1.552907   1  TRUE
1984  5620988  5620988  5622374    4 1.000000 3.308471   4  TRUE
1985  5636445  5636445  5641685    6 1.000000 2.373504   6  TRUE
1986  5644473  5644473  5645556    2 1.000000 1.306303   0  TRUE
1987  5645997  5645997  5647187    4 1.000000 2.559969   4  TRUE
1988  5661697  5661697  5664964    4 1.000000 3.075767   4  TRUE
1989  5677609  5677609  5678791    4 1.000000 4.033083   4  TRUE
1990  5690089  5690089  5691308    4 1.000000 2.158686   2  TRUE
1991  5746106  5746106  5747521    2 1.000000 2.664221   2  TRUE
1992  5884115  5884115  5889196   12 1.000000 2.884591  12  TRUE
1993  5893814  5893814  5897951    6 1.000000 2.037956   4  TRUE
1994  5900207  5900207  5902416    4 1.000000 2.839875   4  TRUE
1995  5995653  5995653  5996783    2 1.000000 2.236852   2  TRUE
1996  6030443  6030443  6032841    6 1.000000 2.611494   6  TRUE
1997  6278433  6278433  6280767    6 1.000000 2.968160   2  TRUE
1998  6281739  6281739  6282412    2 1.000000 3.873618   2  TRUE
1999  6282721  6282721  6285016    6 1.000000 2.968494   6  TRUE
2000  6291836  6291836  6293538    4 1.000000 2.295145   3  TRUE
2001  6334215  6334215  6338195    6 1.000000 2.026189   5  TRUE
2002  6599283  6599283  6601558    4 1.000000 2.460159   3  TRUE
2003  6661944  6661944  6663747    4 1.000000 2.015484   3  TRUE
2004  6694341  6694341  6695681    2 1.000000 2.529447   2  TRUE
2005  6706285  6706285  6708191    2 1.000000 3.518919   2  TRUE
2006  6803417  6803417  6806779   12 1.000000 2.186025  10  TRUE
2007  6854438  6854438  6861966   10 1.000000 2.320646   8  TRUE
2008  6869018  6869018  6870587    4 1.000000 1.798679   2  TRUE
2009  6873392  6873392  6874836    2 1.000000 1.794778   0  TRUE
2010  6978281  6978281  6982912    8 1.000000 2.056908   7  TRUE
2011  6985154  6985154  6990074   12 1.000000 3.974858  12  TRUE
2012  6997050  6997050  6999034    4 1.000000 3.114436   4  TRUE
2013  7095467  7095467  7097637    6 1.000000 3.355950   6  TRUE
2014  7118585  7118585  7123823    6 1.000000 2.049829   3  TRUE
2015  7138091  7138091  7139835    2 1.000000 1.703422   0  TRUE
2016  7147461  7147461  7155264   10 1.000000 2.260718   9  TRUE
2017  7163618  7163618  7167769   10 1.000000 2.961378  10  TRUE
2018  7198945  7198945  7201876    2 1.000000 1.533284   1  TRUE
2019  7223538  7223538  7225754    4 1.000000 2.028722   2  TRUE
2020  7232533  7232533  7234455    4 1.000000 1.808122   4  TRUE
2021  7266233  7266233  7266567    2 0.250000 2.429729   2 FALSE
2022  7273699  7273699  7275992    6 1.000000 3.153510   6  TRUE
2023  7299776  7299776  7301405    2 1.000000 2.333590   2  TRUE
2024  7304959  7304959  7308100    4 1.000000 1.860798   2  TRUE
2025  7317974  7317974  7319013    4 1.000000 2.516386   3  TRUE
2026  7324365  7324365  7325254    2 1.000000 2.204043   2  TRUE
2027  7355466  7355466  7356100    2 1.000000 1.357450   0  TRUE
2028  7459136  7459136  7463293    6 1.000000 2.197181   5  TRUE
2029  7596017  7596017  7596967    4 1.000000 2.305773   2  TRUE
2030  7599372  7599372  7601937    4 1.000000 2.210050   4  TRUE
2031  7612596  7612596  7613044    2 1.000000 2.274450   2  TRUE
2032  7639961  7639961  7642449    2 1.000000 2.216766   2  TRUE
2033  7680492  7680492  7681229    2 1.000000 2.086065   2  TRUE
2034  7696271  7696271  7701942    2 1.000000 2.053748   1  TRUE
2035  7703829  7703829  7707840    6 1.000000 3.623186   6  TRUE
2036  7714305  7714305  7719798   10 1.000000 2.814833  10  TRUE
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2038  7897551  7897551  7899203    4 1.000000 2.255416   4  TRUE
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2182 15970492 15970492 15972417    2 1.000000 2.630847   2  TRUE
2183 16065681 16065681 16068532    4 1.000000 1.733958   2  TRUE
2184 16103743 16103743 16104987    2 1.000000 1.909697   2  TRUE
2185 16220511 16220511 16222276    2 1.000000 2.319154   2  TRUE
2186 16238054 16238054 16238652    2 1.000000 1.633476   1  TRUE
2187 16245005 16245005 16245715    2 0.666667 1.819789   1 FALSE
2188 16254698 16254698 16256821    2 1.000000 1.922479   2  TRUE
2189 16287675 16287675 16289291    2 1.000000 2.008807   1  TRUE
2190 16295209 16295209 16297685    2 1.000000 1.310833   0  TRUE
2191 16318253 16318253 16320090    2 1.000000 2.986810   2  TRUE
2192 16329944 16329944 16334373    2 1.000000 2.494128   2  TRUE
2193 16383749 16383749 16384819    2 1.000000 3.615160   2  TRUE
2194 16423002 16423002 16426825    6 1.000000 2.104588   6  TRUE
2195 16606312 16606312 16608029    2 1.000000 2.810074   2  TRUE
2196 16638747 16638747 16640869    4 1.000000 2.079563   4  TRUE
2197 16643263 16643263 16647585    8 1.000000 1.881574   5  TRUE
2198 16654231 16654231 16655793    2 1.000000 2.287661   1  TRUE
2199 16771501 16771501 16773640    4 1.000000 2.243683   3  TRUE
2200 16782173 16782173 16783136    2 1.000000 1.913017   1  TRUE
2201 16809588 16809588 16810448    2 1.000000 2.127168   1  TRUE
2202 16883605 16883605 16884169    2 0.666667 2.914058   2 FALSE
2203 17108346 17108346 17111037    2 1.000000 2.134677   2  TRUE
2204 17124110 17124110 17126245    4 1.000000 2.232392   3  TRUE
2205 17160404 17160404 17160945    2 1.000000 2.447365   2  TRUE
2206 17163898 17163898 17166543    4 1.000000 3.047885   4  TRUE
2207 17168582 17168582 17171735    2 1.000000 2.376094   2  TRUE
2208 17300851 17300851 17302898    2 1.000000 1.765326   1  TRUE
2209 17404595 17404595 17406841    2 1.000000 2.880907   2  TRUE
2210 17505019 17505019 17505730    2 1.000000 1.962480   2  TRUE
2211 17519404 17519404 17519729    2 0.666667 1.650242   1 FALSE
2212 17522674 17522674 17523791    2 1.000000 3.203637   2  TRUE
2213 17537633 17537633 17540297    6 1.000000 2.067443   4  TRUE
2214 17561895 17561895 17562573    2 1.000000 2.276983   1  TRUE
2215 17577362 17577362 17578622    4 1.000000 2.534578   4  TRUE
2216 17626289 17626289 17628688    6 1.000000 2.212282   3  TRUE
2217 17656418 17656418 17658879    4 1.000000 2.793333   4  TRUE
2218 17699253 17699253 17700010    2 0.666667 1.961039   2 FALSE
2219 17703642 17703642 17704672    2 1.000000 2.522734   2  TRUE
2220 17736538 17736538 17740102    8 1.000000 2.865268   7  TRUE
2221 17740953 17740953 17741168    2 0.333333 3.230672   2 FALSE
2222 17742355 17742355 17742701    2 0.666667 1.697705   1 FALSE
2223 17827412 17827412 17828260    2 0.666667 1.384981   0 FALSE
2224 18515640 18515640 18517113    2 1.000000 1.765322   1  TRUE
2225 18522217 18522217 18527432   10 1.000000 2.293225   8  TRUE
2226 18530024 18530024 18535451   14 1.000000 2.287370  14  TRUE
2227 18549567 18549567 18552058    2 1.000000 2.044773   2  TRUE
2228 18590878 18590878 18591544    2 1.000000 1.849419   1  TRUE
2229 18645668 18645668 18647241    2 1.000000 2.556539   2  TRUE
2230 18700552 18700552 18701386    2 0.666667 2.450852   1 FALSE
2231 19001323 19001323 19002898    2 1.000000 2.018010   2  TRUE
2232 19009701 19009701 19011497    2 1.000000 1.653242   1  TRUE
2233 19013199 19013199 19014945    2 1.000000 2.042527   1  TRUE
2234 19073033 19073033 19074510    4 1.000000 2.533706   4  TRUE
2235 19259908 19259908 19261172    4 1.000000 2.092459   3  TRUE
2236 19353639 19353639 19354676    2 1.000000 1.622793   2  TRUE
2237 19371153 19371153 19371826    2 1.000000 2.743750   2  TRUE
2238 19423139 19423139 19426351    2 1.000000 2.600025   2  TRUE
2239 19430774 19430774 19434556    4 1.000000 1.963896   3  TRUE
2240 19469069 19469069 19469568    2 0.666667 2.044365   1 FALSE
2241 19495598 19495598 19497639    2 1.000000 2.431401   2  TRUE
2242 19508668 19508668 19512283    6 1.000000 1.615169   2  TRUE
2243 19520740 19520740 19521451    2 1.000000 2.304879   2  TRUE
2244 19537121 19537121 19538345    2 1.000000 2.150287   2  TRUE
2245 19555039 19555039 19558855    4 1.000000 2.889909   4  TRUE
2246 19577604 19577604 19579155    2 1.000000 3.024873   1  TRUE
2247 19692928 19692928 19694717    2 1.000000 2.247196   1  TRUE
2248 19717068 19717068 19720972    4 1.000000 1.951301   3  TRUE
2249 19730658 19730658 19731512    2 1.000000 3.223368   2  TRUE
2250 19734431 19734431 19736390    6 1.000000 2.688969   6  TRUE
2251 19743787 19743787 19744204    2 0.666667 2.511750   1 FALSE
2252 19779210 19779210 19780527    2 1.000000 1.769486   1  TRUE
2253 19782810 19782810 19784209    6 1.000000 2.617602   6  TRUE
2254 19785134 19785134 19786535    2 1.000000 1.593646   1  TRUE
2255 19788052 19788052 19788673    2 1.000000 3.533853   2  TRUE
2256 19825330 19825330 19828733    2 1.000000 1.876801   2  TRUE
2257 19842245 19842245 19843731    2 1.000000 2.107969   1  TRUE
2258 19845988 19845988 19851147    8 1.000000 3.017929   8  TRUE
2259 19878066 19878066 19879810    2 1.000000 2.935824   2  TRUE
2260 19888189 19888189 19888663    2 1.000000 2.803774   2  TRUE
2261 19889632 19889632 19890955    2 1.000000 2.444430   2  TRUE
2262 19910596 19910596 19912261    4 1.000000 2.565299   4  TRUE
2263 19916924 19916924 19917537    2 1.000000 2.337820   2  TRUE
2264 20109252 20109252 20109781    2 1.000000 2.057673   1  TRUE
2265 20498974 20498974 20502479    4 1.000000 2.405451   4  TRUE
2266 20552663 20552663 20553674    2 1.000000 1.933296   1  TRUE
2267 20642930 20642930 20646183   10 1.000000 1.622488   2  TRUE
2268 20664622 20664622 20668465    6 1.000000 1.995289   5  TRUE
2269 20717432 20717432 20718035    2 1.000000 1.623155   1  TRUE
2270 20724066 20724066 20726335    6 1.000000 2.571329   6  TRUE
2271 20728397 20728397 20731120    2 1.000000 1.664190   1  TRUE
2272 20747515 20747515 20748921    2 1.000000 2.290085   2  TRUE
2273 20751677 20751677 20752508    2 1.000000 1.663622   1  TRUE
2274 20754775 20754775 20755982    4 1.000000 1.741615   3  TRUE
2275 21052821 21052821 21055674    4 1.000000 2.802712   3  TRUE
2276 21281511 21281511 21282783    2 1.000000 2.070810   2  TRUE
2277 21367097 21367097 21369529    2 1.000000 2.593883   2  TRUE
2278 21598172 21598172 21598796    2 1.000000 1.581359   1  TRUE
2279 22068565 22068565 22070912    2 1.000000 1.821728   1  TRUE
2280 22221706 22221706 22222296    2 1.000000 2.278239   2  TRUE
2281 22454706 22454706 22456974    2 1.000000 3.408969   2  TRUE
2282 22479396 22479396 22481979    4 1.000000 1.958655   3  TRUE
2283 22832217 22832217 22832694    2 1.000000 1.284323   0  TRUE
2284 23401220 23401220 23402427    2 1.000000 2.047473   2  TRUE
2285  1379039  1379039  1380649    2 1.000000 2.002104   1  TRUE
2286  1793673  1793673  1795678    2 1.000000 2.878117   2  TRUE
  • chromosome names are missing

  • add manually - assuming that chromosomes remain in order - 2L, 2R, 3L, 3R, 4, etc

  • first break apart the peaks - the peaks are organised by increasing start site

  • so if the start site is less than the previous peak - new chromosome

  • how many peaks per chromosome?

peaks_per_chr <- vissers_peaks %>% 
  mutate(end_chr = ifelse(start > lead(start), TRUE, NA), 
         row_num = 1:nrow(.), 
         end_chr = ifelse(row_num == nrow(.), TRUE, end_chr),
         end_pos = ifelse(end_chr == TRUE, row_num, NA)) %>% 
  fill(end_pos, .direction = "up") %>% 
  distinct(end_pos) %>% 
  mutate(num_per_chr = end_pos - lag(end_pos), 
         num_per_chr = ifelse(is.na(num_per_chr), end_pos, num_per_chr)) %>%
  .$num_per_chr
peaks_per_chr
[1] 390 421 478 544  39 412   2
  • match to the chromosome - taken from the dm results
chromosomes <- sub("-.*", "", rownames(vissers_dm)) %>% unique()
chromosomes
[1] "chr2L" "chr2R" "chr3L" "chr3R" "chr4"  "chrX"  "chrY" 
  • check that length matches
length(chromosomes) == length(peaks_per_chr)
[1] TRUE
  • replace seqnames
chr_vec <- character()
for(i in 1:length(peaks_per_chr)) {
  chr_vec <- c(chr_vec, rep(chromosomes[i], times = peaks_per_chr[i]))
}

vissers_peaks$seqnames <- chr_vec
head(vissers_peaks)
  seqnames start   end tags      pen aveLogFC sig   big
1    chr2L 17450 21359    6 1.000000 3.888205   5  TRUE
2    chr2L 66423 67818    2 1.000000 2.546664   2  TRUE
3    chr2L 71991 76558    4 1.000000 2.635863   4  TRUE
4    chr2L 93378 95515    4 1.000000 2.727970   4  TRUE
5    chr2L 95762 96492    2 0.666667 2.039309   2 FALSE
6    chr2L 98317 99667    4 1.000000 1.765455   2  TRUE

sessionInfo()
R Under development (unstable) (2024-01-17 r85813)
Platform: x86_64-apple-darwin20
Running under: macOS Sonoma 14.1.1

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRblas.0.dylib 
LAPACK: /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRlapack.dylib;  LAPACK version 3.12.0

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: Australia/Melbourne
tzcode source: internal

attached base packages:
[1] stats4    stats     graphics  grDevices datasets  utils     methods  
[8] base     

other attached packages:
 [1] BSgenome.Dmelanogaster.UCSC.dm6_1.4.1 BSgenome_1.72.0                      
 [3] rtracklayer_1.64.0                    BiocIO_1.14.0                        
 [5] Biostrings_2.72.1                     XVector_0.44.0                       
 [7] ggplot2_3.5.1                         edgeR_4.2.1                          
 [9] limma_3.60.4                          tidyr_1.3.1                          
[11] dplyr_1.1.4                           plyranges_1.24.0                     
[13] GenomicRanges_1.56.1                  GenomeInfoDb_1.40.1                  
[15] IRanges_2.38.1                        S4Vectors_0.42.1                     
[17] BiocGenerics_0.50.0                   Damsel_1.0.0                         
[19] workflowr_1.7.1                      

loaded via a namespace (and not attached):
 [1] tidyselect_1.2.1            bitops_1.0-8               
 [3] fastmap_1.2.0               RCurl_1.98-1.16            
 [5] GenomicAlignments_1.40.0    promises_1.3.0             
 [7] XML_3.99-0.17               digest_0.6.35              
 [9] lifecycle_1.0.4             statmod_1.5.0              
[11] processx_3.8.4              magrittr_2.0.3.9000        
[13] compiler_4.4.0              rlang_1.1.3                
[15] sass_0.4.9                  tools_4.4.0                
[17] utf8_1.2.4                  yaml_2.3.8                 
[19] knitr_1.46                  S4Arrays_1.4.1             
[21] curl_5.2.1                  DelayedArray_0.30.1        
[23] abind_1.4-5                 BiocParallel_1.38.0        
[25] withr_3.0.1                 purrr_1.0.2                
[27] grid_4.4.0                  fansi_1.0.6                
[29] git2r_0.33.0                colorspace_2.1-1           
[31] scales_1.3.0                SummarizedExperiment_1.34.0
[33] cli_3.6.2                   rmarkdown_2.27             
[35] crayon_1.5.3                generics_0.1.3             
[37] rstudioapi_0.16.0           httr_1.4.7                 
[39] rjson_0.2.21                cachem_1.1.0               
[41] stringr_1.5.1.9000          splines_4.4.0              
[43] zlibbioc_1.50.0             parallel_4.4.0             
[45] BiocManager_1.30.23         restfulr_0.0.15            
[47] matrixStats_1.3.0           vctrs_0.6.5                
[49] Matrix_1.7-0                jsonlite_1.8.8             
[51] callr_3.7.6                 locfit_1.5-9.10            
[53] jquerylib_0.1.4             glue_1.7.0                 
[55] codetools_0.2-20            ps_1.7.6                   
[57] stringi_1.8.4               gtable_0.3.5               
[59] later_1.3.2                 UCSC.utils_1.0.0           
[61] munsell_0.5.1               tibble_3.2.1               
[63] pillar_1.9.0                htmltools_0.5.8.1          
[65] GenomeInfoDbData_1.2.12     R6_2.5.1                   
[67] rprojroot_2.0.4             evaluate_0.23              
[69] Biobase_2.64.0              lattice_0.22-6             
[71] Rsamtools_2.20.0            renv_1.0.7                 
[73] httpuv_1.6.15               bslib_0.7.0                
[75] Rcpp_1.0.12                 SparseArray_1.4.8          
[77] whisker_0.4.1               xfun_0.44                  
[79] fs_1.6.4                    MatrixGenerics_1.16.0      
[81] getPass_0.2-4               pkgconfig_2.0.3